Journal: Saudi journal of biological sciences

Article Title: Phytochemical profiling, antioxidant and antiproliferation potential of Euphorbia milii var.: Experimental analysis and in-silico validation.

doi: 10.1016/j.sjbs.2020.08.003

Figure Lengend Snippet: Fig. 2. Effect of E. milii on mRNA expression of specific gene of cell cycle regulators. (A) qRT-PCR was performed to assess the mRNA levels of cells regulators genes. HepG2 cells were cultured in the presence or absence of 11.2 mM E. milii water extract for 24 h. The GAPDH was used as internal positive control. The data for E. milii-treated samples were presented as the mean ± SEM of triplicate determination and compared with the control group. *p < 0.05; **p < 0.01. (B) Effect of E. milii on expression of cell cycle regulators. The cells were treated with or without E. milii for indicated time. The expression of CDK2, E2F1 and cyclin E were determined with western blot analysis.

Article Snippet: The nitrocellulose membranes were put into the blocking solution (5% fat free milk) for 1 h, then incubated the monoclonal anti-rabbit CDK2, cyclin E, E2F1 and anti-b-actin with shaking over night at 4 C. The horseradish peroxidase (HRP)conjugated secondary antibody given for at least 1 h, then chemiluminescence was detected using a chemiluminescence imaging system (Bio-Rad, Hercules, CA).

Techniques: Expressing, Quantitative RT-PCR, Cell Culture, Positive Control, Control, Western Blot

Journal: Saudi journal of biological sciences

Article Title: Phytochemical profiling, antioxidant and antiproliferation potential of Euphorbia milii var.: Experimental analysis and in-silico validation.

doi: 10.1016/j.sjbs.2020.08.003

Figure Lengend Snippet: Fig. 4. Obtained binding modes of ligands in the binding domain of CDK2 (magenta) and Thymidlate synthase (skyblue). (A) Binding mode of compound BAN in CDK2 ATP binding site. (B) Compound CBT bonded to CDK2. (C) Docking complex of CDK2-MBT. Binding mode of selected compounds in the active site of Thymidlate synthase (TS): (D) BAN-TS, (E) CBT-TS, (F) MBT-TS and (G) compound 5-FU (standard) bonded to TS.

Article Snippet: The nitrocellulose membranes were put into the blocking solution (5% fat free milk) for 1 h, then incubated the monoclonal anti-rabbit CDK2, cyclin E, E2F1 and anti-b-actin with shaking over night at 4 C. The horseradish peroxidase (HRP)conjugated secondary antibody given for at least 1 h, then chemiluminescence was detected using a chemiluminescence imaging system (Bio-Rad, Hercules, CA).

Techniques: Binding Assay

Journal: Saudi journal of biological sciences

Article Title: Phytochemical profiling, antioxidant and antiproliferation potential of Euphorbia milii var.: Experimental analysis and in-silico validation.

doi: 10.1016/j.sjbs.2020.08.003

Figure Lengend Snippet: Fig. 5. RMSDs of Ca atoms of the protein, backbone atoms of binding pocket (within 6.5 Å), and the heavy atoms in the ligand for: (A) CDK2-BAN, (B) CDK2-CBT, (C) TS-CBT and (D) TS-5-FU. Comparison between binding free energy terms of: (E) CDK2 bonded to BAN and CBT; (F) TS bonded to CBT and 5-FU.

Article Snippet: The nitrocellulose membranes were put into the blocking solution (5% fat free milk) for 1 h, then incubated the monoclonal anti-rabbit CDK2, cyclin E, E2F1 and anti-b-actin with shaking over night at 4 C. The horseradish peroxidase (HRP)conjugated secondary antibody given for at least 1 h, then chemiluminescence was detected using a chemiluminescence imaging system (Bio-Rad, Hercules, CA).

Techniques: Binding Assay, Comparison